Teknik Konsentrasi Formol Eter untuk Mendiagnosa Parasit Usus

Elva Susanty

Abstract


Infeksi parasit usus merupakan penyakit endemik yang menyebabkan masalah kesehatan di negara berkembang seperti Indonesia. Diagnosa parasit usus memerlukan teknik pemeriksaan yang sensitif dan biaya murah. Teknik konsentrasi formol eter (formol eter concentration/FEC) merupakan salah satu teknik pemeriksaan dengan sampel feses untuk mendeteksi parasit usus dengan prinsip sedimentasi. Tehnik ini merupakan salah satu tehnik yang ekonomis, mudah dilakukan, dan meningkatkan jumlah penemuan parasit usus karena dapat memisahkan debris dari feses. Tujuan: Mengetahui teknik konsentrasi formol eter dalam mendiagnosa parasit usus. Tinjauan Pustaka: Prevalensi infeksi parasit usus masih cukup tinggi di negara berkembang akibat kurangnya sanitasi dan buruknya higiene, sehingga diperlukan suatu teknik pemeriksaan yang bisa mendeteksi kista protozoa, larva, dan telur cacing. Teknik FEC menggunakan formalin 10% dalam air sebagai penstabil dan eter sebagai pelarut. Teknik FEC tidak merusak organisme dalam sampel feses, dapat mengetahui parasit usus dengan sampel feses sedikit, dan ekonomis. Beberapa penelitian menunjukkan bahwa teknik FEC lebih baik daripada teknik direct wet mount dalam mendiagnosa parasit usus. Simpulan:  Teknik FEC merupakan salah satu teknik pemeriksaan yang sensitif dan ekonomis dan dapat digunakan sebagai pilihan untuk mendiagnosa parasit usus.

Keywords


parasit usus; sedimentasi; teknik diagnosa; teknik konsentrasi formol eter

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References


Haque R. Human intestinal parasites. J Health Popul Nutr. 2007;24(4):387-91.

Amin HA, Ali SA. Evaluation of different techniques of stool examination for intestinal parasitic infections in Sulaimani City – Iraq. Int. J. Curr. Microbiol. App.Sci. 2015;4(5):991-96.

Venkatesh BMS, Rao SR, Vivekanand N, Shanker BM. A comparative study of concentration techniques for detection of intestinal parasitic infections – to evaluate the prevalence and to identify a better method of concentration technique at aTribal Tertiary Care Hospital. Journal of Dental and Medical Science. 2016;15(7):42-6.

Becker SL, Lohourignon LK, Speich B, Rinaldi L, Knopp S, Goran KE, et al. Comparison of the FLOTAC-400 dual technique and the formalin ether concentration technique for diagnosis of human intestinal protozoon infection. J. Clin. Microbiol. 2011;9(6):2183-90.

Balakrishna J, Venkateswarlu, Bai K, Saheb HS. Comparison of modified 1% Potassium Hydroxide Formol-Ether concentration technique with direct wet mount preparation and standard formol-ether concentration technique for detection of parasites in stool. J.Pharm. Sci. & Res. 2013;5(7):147-49.

Kassa D, Mengestie A, Ayalew N, Belaynew A, Mebrie Z, Metadel T. A Review on diagnostic techniques in veterinary helminthlogy. Nat Sci. 2016;14(7):109-118]. ISSN 1545-0740 (print); ISSN 2375-7167 (online). http://www.sciencepub.net/nature. 15. doi:10.7537/marsnsj140716.15.

McHardy IH, Wu M, Cohen RS, Couturier MR, Humpries RM. Detection of Intestinal Protozoa in the Clinical Laboratory. Journal of Microbiology. 2014;52(3):712-20.

Pakdad K, Nasab SD, Damraj FA, Ahmadi NA. Comparing the efficiency of four diagnostic concentration techniques performed on the same group of intestinal parasites. Alex J Med. 2017. Available at: https://doi.org/10.1016/j.ajme.2017.11.005.

Nikolay B, Brooker SJ, Pullan RL. Sensitivity of diagnostic test for human soil-transmitted helminth infections: a meta-analysis in the absence of a true gold standard. Int J Parasitol. 2014;44(11):765-74.

Oguoma V, Ekwunife C. The need for a better method: comparison of direct smear and formol-ether concentration techniques in diagnosing intestinal parasites. The internet journal of tropical. 2006;3(2):1-6.

Frische TR, Pritt BS. Medical Parasitology. Dalam: McPherson RA, Pincus MR. edisi ke-23. Henry's Clinical Diagnosis and Management by Laboratory Methods. St. Louis, Missouri: Elsevier, 2017.Chapter 63, 1231-83.

Parameshwarappa KD, Chandrakanth C., Sunil B. The prevalence of intestinal parasitic infestations and the evaluation of different concentration techniques of the stool examination. Journal of Clinical and Diagnostic Research. 2012;6(7):1188-91.

Frische TR, Pritt BS. Medical Parasitology. Dalam: McPherson RA (penyunting), Pincus MR (Penyunting). Edisi ke-23. Henry's Clinical Diagnosis and Management by Laboratory Methods, St. Louis, Missouri: Elsevier, 2017.Chapter 63, 1231-1283.e3.

Manser MM, Saez ACS, Chiodini PL. Faecal parasitology: concentration methodology needs to be better standardised. PLoS Negl Trop Dis. 2016;10(4):1-16.

Sato C, Rai SK, Uga S. Re-evaluation of the formalin-ether sedimentation method for the improvement of parasites egg recovery efficiency. Nepal Med Coll. 2014;16(1):20-25.

World Health Organization (WHO). Manual of Basic Techniques for a Health Laboratory. Alih bahasa: Chairlan., Lestari Estu. Editor edisi Bahasa Indonesia: Albertus Agung Mahode. Pedoman Teknik Dasar untuk Laboratorium Kesehatan. edisi ke 2. Penerbit Buku Kedokteran EGC, Jakarta;2011. p. 150-52.

Endris M, Tekeste Z, Lemma W, Kassu A. Comparison of the Kato-Katz, Wet Mount, and Formol-Ether concentration diagnostic techniques for intestinal helminth infections in Ethiopia. ISRN. Parasitology. 2013, Article ID 180439, 5 pages http://dx.doi.org/10.5402/2013/180439.

Lier T, Simonsen GS, Wang T, Lu D, Haukland HH, et al. Low sensitivity of the Formol-Ethyl Acetate sedimentation concentration technique in low-Intensity Schistosoma japonicum infections. PLoS Negl Trop Dis. 2009;3(2):e386. doi: 10.1371/journal.pntd.0000386.

Taye, S. Comparison of Kato-Katz and Formol Ether Concentration methods for the diagnosis of intestinal helmintic infection among school children of Wonji Shoa town, Eastern Ethiopia: a school based cross sectional study. American Journal of Health Research. 2014;2(5):271-74.

Hussien M, Alamin EA. A comparison between the efficiency of formal gasoline concentration technique and other techniques used for the detection of intestinal parasites. International Journal of Preclinical & Pharmaceutical Research. 2015;6(2):91-4.

Dryden MW, Payne PA. Fecal examination techniques. NAVC Clinician’s Brief. 2010:13-16.

Moges F, Belyhun Y, Tiruneh M, Kebede Y, Mulu A, et al. Comparison of formol-acetone concentration method with that of the direct iodine preparation and formol-ether concentration methods for examination of stool parasites. Ethiop. J. Health Dev. 2010;24(2):148-51.




DOI: https://doi.org/10.26891/jkm.v1i2.2018.125-129

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